Application of calcium peroxide in promoting resource recovery from municipal sludge: A review.

The harmless disposal, resource recovery, and synergistic efficiency reduction of municipal sludge have been the research focuses for the last few years. Calcium peroxide (CaO2) is a multifunctional and safe peroxide that produces an alkaline oxidation environment to promote the fermentation of municipal sludge to produce hydrogen (H2) and volatile fatty acids (VFAs), thus realizing sludge resource recovery. This review outlines the research achievements of CaO2 in sludge resource recovery, improvement of sludge dewaterability, and removal of pollutants from sludge in recent years. Meanwhile, the mechanism of CaO2 and its influencing factors have also been comprehensively summarized. Finally, the future development direction of the application of CaO2 in municipal sludge is prospected. This review would provide theoretical reference for the potential engineering applications of CaO2 in improving sludge treatment in the future.

Oxidative stress responses in two marine diatoms during sulfamethoxazole exposure and the toxicological evaluation using the IBRv2 index.

Sulfamethoxazole (SMX) is widely present in water systems, and its stable properties and poor biodegradability can result in high residues of SMX in the water environment. This, in turn, can have detrimental effects on the entire aquatic habitat and human life and health. This study aimed to investigate the toxic effects of SMX on the growth, photosynthetic pigment content, and oxidative stress of two marine microalgae species: Skeletonema costatum and Phaeodactylum tricornutum. SMX demonstrated a significant inhibitory effect on microalgae proliferation, with 96-h median effective concentration (EC50) values of 0.93 mg/L and 4.65 mg/L for S. costatum and P. tricornutum, respectively. At low concentrations, SMX significantly increased the production of Chl a in both microalgae species. However, in the higher concentration SMX treatment group, Chl a content in P. tricornutum experienced a significant decrease, whereas Chl c showed no sensitivity to SMX. The activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx), along with the glutathione (GSH) content, exhibited a significant increasing trend in response to higher SMX concentrations. However, these changes effectively inhibited the accumulation of malondialdehyde (MDA) content. In the treatment group with the highest SMX concentration, MDA content in both microalgae species was significantly higher compared to the control group. The Integrated Biomarker Response Version 2 (IBRv2) index showed a significant positive correlation with SMX concentration, suggesting its potential for assessing the ecotoxicological effects of lower SMX concentrations on marine microalgae.

Effects of o-cresol on the growth and biochemical compositions in marine microalgae.

To assess the toxic effects of o-cresol on marine organisms, Skeletonema costatum and Phaeodactylum tricornutum were chosen as test subjects to investigate its impact on growth and biochemical compositions. The results indicated that the 96-h EC50 values for o-cresol in S. costatum and P. tricornutum were 7.99 mg/L and 13.28 mg/L, respectively, demonstrating a moderate and slight toxicity level. Conversely, the maximum no-effect concentration (NOEC) for o-cresol in S. costatum and P. tricornutum were 2.43 mg/L and 0.43 mg/L, respectively, classifying their chronic toxicity grades as negligible and low toxic. Following a 96-h exposure period, the content of photosynthetic pigments in S. costatum did not significantly differ from the control group (P > 0.05). Conversely, the levels of total protein, total lipid, and carbohydrate in microalgae were significantly induced (P < 0.05) as the concentration of o-cresol increased. Higher concentrations of o-cresol generally stimulated the synthesis of biochemical compositions in algae cells, which serves as an active defense mechanism in response to pollution stress. To comprehensively evaluate the potential risk of o-cresol to marine ecosystems, it is crucial to strengthen its toxicity studies on marine fish and crustaceans in the future.

Oxidative stress responses in two marine diatoms during acute n-butyl acrylate exposure and the toxicological evaluation with the IBRv2 index.

n-Butyl acrylate (nBA), a typical hazardous and noxious substance (HNS), is the largest-volume acrylate ester used to produce various types of polymers. With the increasing volume of nBA subject to maritime transportation, its accidental leakage poses a great risk to the marine organisms. Therefore, it is necessary to evaluate the ecological risk of nBA in marine environments. In this study, two species of marine microalgae, Skeletonema costatum and Phaeodactylum tricornutum, were used to explore the toxic effects of nBA based on their growth, pigment content, and oxidative stress. The growth of each species was significantly inhibited by nBA, showing a 96 h-EC50 value of 2.23 mg/L for P. tricornutum and 8.19 mg/L for S. costatum, respectively. Although chlorophylls a and c exerted a hormesis effect in P. tricornutum, contents of pigments generally decreased at high concentrations. In P. tricornutum, all detected antioxidants (reduced glutathione, GSH; superoxide dismutase, SOD; catalase, CAT; and glutathione peroxidase, GPx) were stimulated at concentrations ranging from 1.50 to 3.82 mg/L. However, these elevations were not enough to reduce the oxidative damage caused by nBA, because the content of malondialdehyde (MDA) increased continuously during 96-h exposure. For S. costatum, the activities of only two antioxidants (GSH and CAT) were enhanced, which is enough to prevent the MDA content from rising, even at higher concentrations of nBA (5-10 mg/L). The Integrated Biomarker Response Version 2 (IBRv2) index that combines responses of the above five oxidative stress biomarkers, was not only correlated positively with nBA concentration but could also indicate the occurrence of oxidative stress caused by acute concentration of nBA. These findings showed that P. tricornutum was sensitive to nBA compared to S. costatum, and the IBRv2 index was an effective tool for evaluating ecotoxicological effects on marine microalgae due to nBA spills.

Immobilization of Ochrobactrum sp. on Biochar/Clay Composite Particle: Optimization of Preparation and Performance for Nitrogen Removal.

The objective of this study was to prepare biochar/clay composite particle (BCCP) as carrier to immobilize Ochrobactrum sp. to degrade ammonia nitrogen (NH4 +-N), and the effects of calcined program and immobilizing material were investigated. Results reflected that the parameters were as follows: calcined temperature 400°C, heating rate 20°C min-1, and holding time 2 h, and the adsorption capacity could reach 0.492 mg g-1. Sodium alginate/polyvinyl alcohol, as embedding material, jointed with NH4 +-N adsorption process and then degraded by Ochrobactrum sp. with 79.39% degradation efficiency at 168 h. Immobilizing Ochrobactrum sp. could protect strain from high salt concentration to achieve the exceeding degradation efficiency than free bacteria, but could not block the impact of low temperature.

Occurrence and ecotoxicity of sulfonamides in the aquatic environment: A review.

Rapid population growth and increasing demand for animal protein food have led to a continuous increase in global utilization of antibiotic. Sulfonamides (SAs) are ubiquitous in aquatic environments and pose an ecological risk owing to their large consumption and strong environmental persistence. Hence, this review focuses on the recent publications on 12 different SAs and provides a detailed summary of selected antibiotic concentrations in various water systems. We evaluated the ecotoxicity of SAs on organisms at different trophic level organisms and the environmental risks regarding aquatic systems. The results indicated that SA antibiotics were ubiquitous in aquatic environments at concentrations ranging from ng/L to µg/L. According to the data using standard ecotoxicity bioassays, algae were the most susceptible aquatic organisms for selected antibiotics, followed by crustaceans and fish. The risk data suggested that some antibiotics, such as sulfadiazine (SDZ), sulfamethoxazole (SMX), and sulfamethazine (SMZ) pose a great risk to the aquatic system. Based on the present review, it is necessary to strengthen the research into their ecotoxicity to marine systems and the chronic toxicity of antibiotic mixtures.

The pathways by which the marine diatom Thalassiosira sp. OUC2 biodegrades p-xylene, combined with a mechanistic analysis at the proteomic level.

A marine diatom, Thalassiosira sp. OUC2, was isolated from natural seawater collected from Daya Bay, China. This diatom degraded 1.25-40 mg L-1p-xylene within five days, at a removal efficiency exceeding 98%. Gas chromatography-mass spectrometer (GC-MS) analysis indicated that p-xylene was converted into 4-methylbenzyl alcohol, p-toluic acid, and p-cresol in the presence of strain OUC2. Meanwhile, proteomic analysis showed that, after exposure to p-xylene, several algal enzymes were significantly upregulated: including monooxygenase, alcohol dehydrogenase, benzaldehyde dehydrogenase, benzoate 1,2-dioxygenase, and catechol 2,3-dioxygenase. Moreover, ecotoxicological tests suggested that the intermediate metabolites were less toxic than the parent compound (p-xylene). Thalassiosira sp. OUC2 may thus be suitable for the remediation of p-xylene-contaminated marine environments.

Ecotoxicity of chlorpyrifos to aquatic organisms: A review.

Pesticides play an important role in promoting agricultural development, while their unreasonable use has led to environmental problems. Chlorpyrifos (CPF), a typical organophosphate pesticide, is used globally as an insecticide in agriculture. The extensive application of CPF has resulted in water contamination, and CPF has been detected in rivers, lakes, seawater, and even in rain. In the present review, CPF was selected due to its extensive use in agriculture and higher detection rate in surface waters. In this review we summarised the evidence related to CPF pollution and focused on discussing the ecotoxicity of CPF to aquatic systems and revealed the mechanism of action of CPF. The aim of this literature review was to summarise the knowledge of the toxicity to marine and freshwater organisms of CPF as well as try to select a series of sensitive biomarkers, which are suitable for ecotoxicological assessment and environmental monitoring in aquatic systems.

Biological nitrogen removal and metabolic characteristics in a full-scale two-staged anoxic-oxic (A/O) system to treat optoelectronic wastewater.

In order to explore the treatment efficiency of optoelectronic wastewater and pollutant degradation mechanism of full-scale two-stage AO process, 160 d monitoring was conducted in this study. The results showed that the two-stage AO process owned relatively stable nitrogen and organic matter removal performance. The average concentration of COD, NH4+-N, and TN in effluent was 54, 3.78 and 13.77 mg L-1, respectively, and the removal rate was over 80%. The results of high-throughput sequencing demonstrated that the dominant microorganism was Proteobacteria, Bacteroidetes, Firmicutes, Chlorofeli, and Acidobacteria, and differences of interaction networks exited between aerobic and anoxic units. Meanwhile, the microorganism metabolism in aerobic units was significantly different from that in anoxic unit, and the metabolism of the microbial community for treating optoelectronic wastewater was significantly different from that for treating urban domestic sewage.

Biodegradation of phenol in saline or hypersaline environments by bacteria: A review.

With the continuous demand from industry for chemical raw materials, a large amount of high-salinity wastewater containing phenol is discharged into the aquatic environment, and the leakage of dangerous chemicals into the sea may lead to phenol pollution of the ocean. Phenol is a common chemical posing serious environmental hazard. Biodegradation is an effective, low-cost, environment-friendly method of removing phenol from water, but in hypersaline environments, traditional freshwater organisms are less efficacious. Here, at least 17 genera of bacteria from three phyla are found that can degrade phenol in different saline environments. The sources and taxonomy of halotolerant and halophilic bacteria are reviewed. Moreover, the pathway of phenol removal, kinetics of biodegradation, influencing factors, and recent treatment processes of wastewater are discussed.

Toxicological Assessment of Intermediates in Natural Attenuation of p-Xylene to Marine Microalgae.

The toxic effects of p-xylene, 4-methylbenzyl alcohol, p-methyl benzaldehyde, and p-toluic acid on two marine microalgae (Phaeodactylum tricornutum Bohlin, and Skeletonema costatum) were investigated. p-Xylene was the most toxic to Pha. tricornutum with a 96 h EC50 value of 6.76 mg L-1. Based on the 96 h EC50 values for two microalgae, the toxicity of the four chemicals, in descending order, was: p-xylene, p-methyl benzaldehyde, 4-methylbenzyl alcohol, then p-toluic acid. The results showed that the toxicity of the transformed products of p-xylene was lower than that of p-xylene.

Kinetic analysis and degradation mechanism for natural attenuation of xylenes under simulated marine conditions.

Microcosm experiments were conducted to examine the attenuation of selected chemicals, i.e. m-xylene (MX), o-xylene (OX) and p-xylene (PX), under simulated marine conditions. Natural attenuation and the contribution of oxidation, photodegradation, biodegradation and volatilization to total attenuation were evaluated. The development of attenuation was in agreement with pseudo-first-order kinetics for all xylenes. The half-lives of MX, OX, and PX under optimal conditions were 0.76, 0.74 and 0.88 days, respectively. Attenuation kinetics were proposed to analyze the natural attenuation of xylenes. The leading attenuation type of MX, OX, and PX was volatilization, and the attenuation rate constants (KV) were 0.5587, 0.6733, and 0.4821 d-1, respectively. Biodegradation of OX (Kb: 0.0003 d-1) was extremely inhibited. The attenuation kinetics presented the attenuation of xylenes in microcosm. The reaction kinetics could be applied to analyze the natural attenuation of chemicals. MX and OX can be converted to one another under certain conditions. Toluene and ethylbenzene were detected for OX in the OP (oxidation and photodegradation) experiment under simulated marine conditions. 4-Methylbenzyl alcohol, p-methyl benzaldehyde and p-toluic acid, as the major intermediates, were identified during the natural attenuation of PX using GC/MS.

Ecotoxicity of phenol and cresols to aquatic organisms: A review.

With the development of industrial production and continuous demand for chemicals, a large volume of wastewater containing phenols was discharged into the aquatic environment. Moreover, chemical leakage further increased the emission of phenols into aquatic systems. Phenol and its methylated derivative (cresols) were selected due to their extensive use in industry and ecotoxicity to freshwater and marine organisms. This review focused on the ecotoxicity of phenol and m-, o-, and p-cresol on aquatic systems. The mechanism of action of phenols was also discussed. The aim of this literature review was to summarise the knowledge of the behaviour, and toxicity on marine and freshwater organisms, of phenols as well as to try to select a series of sensitive biomarkers suitable for ecotoxicological assessment and environmental monitoring in aquatic environments.

Two-stage mixotrophic cultivation for enhancing the biomass and lipid productivity of Chlorella vulgaris.

This study proposes a two-stage mixotrophic process for cultivating Chlorella vulgaris. Heterotrophic growth is the dominant step in Phase I (to increase microalgal biomass) and photoautotrophic growth occurs in Phase II (to improve biomass concentration and lipid production). The results show that the addition of the low-cost antioxidant sodium erythorbate (8 g L-1) significantly accelerates the growth of microalgae in the first stage with air aeration. Furthermore, a higher CO2 fixation rate was obtained in the second stage (at least 344.32 mg CO2 L-1 day-1) with 10% CO2 aeration. This approximately corresponds to an increase of 177% over simple photoautotrophic cultivation with 10% CO2 aeration during the whole period. The two-stage cultivation strategy achieved a maximum C. vulgaris biomass concentration of 3.45 g L-1 and lipid productivity of 43.70 mg L-1 day-1, which are 1.85 and 1.64 times those arising due to simple photoautotrophy, respectively. Moreover, an analysis of the product's fatty acid profile indicates that C. vulgaris might be an ideal candidate for two-stage mixotrophic cultivation of a renewable biomass for use in biodiesel applications. Another interesting point to note from the study is that it is an insufficiency of N and CO2 that probably limits the further growth of C. vulgaris.

Environmental behavior and eco-toxicity of xylene in aquatic environments: A review.

With the demand for chemicals and fuels increasing continuously, the occurrence of accidental leakage poses great risks to the aquatic environment. Xylene, a hazardous and noxious substance, has been major concerns with regard to heterogeneity and eco-toxicity towards aquatic organisms. This review focused on the ecotoxicological hazards of m-, o-, and p-xylene, as well as mixed xylene, on aquatic organisms. The mechanism of action of xylenes was also demonstrated in details. The purpose of this review was to further understand transfer and diffusion of toxicity on marine and freshwater organisms of xylene in the aquatic environment. Another aim was to screen sensitive biomarkers which were suitable for ecotoxicological assessment and monitoring in an aquatic system.

Four types of attenuation of phenol and cresols in microcosms under simulated marine conditions: A kinetic study.

Phenol, o-cresol, m-cresol, and p-cresol were selected to conduct microcosm experiments to examine their attenuation under simulated marine conditions, aiming at estimating natural attenuation and the contribution of oxidation, photolysis, biodegradation, and volatilisation to total attenuation of phenol and three cresols in the marine environment. The development of attenuation in microcosms showed the relevance of the pseudo-first-order kinetic for all phenols. The half-lives of phenol, o-cresol, m-cresol, and p-cresol attenuation under optimal conditions were 7.9, 4.3, 5.3, and 4.4 d, respectively. Attenuation kinetics was proposed to analyse the natural attenuation of phenol and cresols. The leading attenuation type of phenol, o-cresol, and p-cresol was volatilisation, and the attenuation rate constants (Kv) were 0.0356, 0.0687, and 0.0710 d-1. Photolysis (Kp: 0.0584 d-1) was the major attenuation type for m-cresol. Biodegradation of phenol (Kb: 0.0021 d-1) and m-cresol (Kb: 0.0049 d-1) were extremely inhibited. The rank between the contributions of the four types of attenuation to total attenuation differed between phenol and the three cresols. The attenuation kinetics proposed in this study possibly demonstrated the attenuation of the phenol and cresols in microcosm. This new reaction kinetics can be used in the analysis of natural attenuation of chemical substances.

Toxicological effects of phenol on four marine microalgae.

The toxic effects of phenol on four marine microalgae (Dunaliella salina, Platymonas subcordiformis, Phaeodactylum tricornutum Bohlin, and Skeletonema costatum) were evaluated. The 96h EC50 values were 72.29, 92.97, 27.32, and 27.32mgL-1, respectively, which were lower than those values of freshwater microalgae reported in the literature. During a 96-h exposure to a sub-lethal concentration of phenol (1/2 96h EC50) with green alga (D. salina) and diatom (S. costatum), reactive oxygen species (ROS) accumulation, and chlorophyll a (Chl a) content decrease were simultaneously observed in diatom cells after 48h treatment. On the contrary, other chlorophylls in both algae were unaffected. Under transmission electron microscopy (TEM), the phenol-induced ultrastructure alterations included disappearance, or shrinkage, of nucleolus and enlargement of vacuoles, which may result in programmed cell death (PCD). The increase in number of lipid droplets may be related to phenol detoxification. These results indicate that the sensitivity of marine microalgae to phenol was dependent on some biotic factors such as cell size, ROS production, and phenol degradation ability in algal cells.